SUSHI Applications


NameAnalysis CategoryDescriptionRequired ColumnsNext DataSet Columns
AtacENCODEApp A ATAC-seq and DNase-seq processing pipeline from ENCODE.
Name, Read1, Read2, Species Name, Report [File], Stats [File], Html [Link]
HomerDiffPeaksApp Finding Peaks and Differential Peaks with or without Replicates from HOMER.
Name, BAM Name, Report [File], DiffPeak [Link]
ProdigalApp Prodigal: rapid prokaryotic genome annotation Name, Draft Name, Out [File], Proteins [File], Species
ProkkaApp Prokka: rapid prokaryotic genome annotation Name, Draft Name, ProkkaOut [File], Species
PsortbApp Psortb: subcellular protein localization prediction tool for Bacteria and Archea Name, Proteins Name, PsortbOut [File], Species
CanuApp Canu long read genome assembler Name, Read1 Name, Reads, Draft [File]
SpadesApp SPAdes genome assembler Name, Read1 Name, Draft [File], SpadesLog [File], PreprocessingLog [File], Species, Read Count
UnicyclerApp Unicycler microbial genome assembler Name, Read1 Name, Draft [File], Graph [File], Log [File], Species, Read Count
ExceRptApp Annotation and Profiling of smallRNA-seq with exceRpt's pipeline
Name, Read1, Adapter1, Species Name, excerpt [File], Species, refBuild
FeatureCountsApp Multi-purpose read counting with Rsubread::featureCounts
Name, BAM, BAI, refBuild Name, Count [File], Stats [File], Species, refBuild, featureLevel, refFeatureFile, strandMode, paired, Read Count, transcriptTypes
KallistoApp kallisto is a program for quantifying abundances of transcripts from RNA-Seq data. It is based on the novel idea of pseudoalignment for rapidly determining the compatibility of reads with targets, without the need for alignment. Name, Read1, Species Name, Count [File], bootstrappedCount [File], runInfo [File], PreprocessingLog [File], Species, refBuild, featureLevel, refFeatureFile, strandMode, paired, Read Count, transcriptTypes
DESeq2App Differential gene expression analysis based on the negative binomial distribution
Name, Count, Species, refBuild, featureLevel, refFeatureFile Name, Species, refBuild, Static Report [Link], Live Report [Link], Report [File]
EdgeRApp Empirical analysis of digital gene expression data in R
Name, Count, Species, refBuild, featureLevel, refFeatureFile Name, Species, refBuild, Static Report [Link], Live Report [Link], Report [File]
LimmaApp Empirical analysis of digital gene expression data in R
Name, Count, Species, refBuild, featureLevel, refFeatureFile Name, Species, refBuild, Static Report [Link], Live Report [Link], Report [File]
BWAApp Burrows-Wheeler Aligner
Name, Read1, Species Name, BAM [File], BAI [File], IGV [Link], Species, refBuild, paired, refFeatureFile, strandMode, Read Count, IGV [File], PreprocessingLog [File]
BismarkApp A tool to map bisulfite converted sequence reads and determine cytosine methylation states
Name, Read1, Species Name, BAM [File], BAI [File], TxtReport [File], M-Bias_R1 [File], M-Bias_R2 [File], CpG_Context [File], COV [File], BedGraph [File], Species, refBuild, paired, Read Count, PreprocessingLog [File]
Bowtie2App Fast and sensitive read alignment. Supports local and end-to-end mode
Name, Read1, Species Name, BAM [File], BAI [File], IGV [Link], Species, refBuild, paired, Read Count, IGV [File], PreprocessingLog [File], Bowtie2Log [File]
Minimap2App https://lh3.github.io/minimap2/minimap2.html Name, Read1, Species Name, BAM [File], BAI [File], IGV [Link], Species, refBuild, paired, refFeatureFile, strandMode, Read Count, IGV [File], PreprocessingLog [File]
Pbmm2App A minimap2 frontend for PacBio native data formats
Name, Read1 Name, BAM [File], BAI [File], IGV [Link], refBuild, IGV [File], Pbmm2Log [File]
STARApp Ultafast spliced alignment
Name, Read1, Species Name, BAM [File], BAI [File], IGV [Link], Species, refBuild, paired, refFeatureFile, strandMode, Read Count, IGV [File], PreprocessingLog [File], STARLog [File]
KrakenApp Kraken taxonomic sequence classification system Name, Read1 Name, KronaReport [Link], KrakenOut [File], KrakenReport [File], KronaOutDir [File], KronaOut [File]
PostSamsa2AnalysisApp Step_6 of Samsa2, data analysis and report. Name, annotationORGFileRefSeq, annotationFUNCFileRefSeq Name, Report [File], Static Report [Link]
QIIME2App Data processing with QIIME2. For short reads/Illumina data only. Name, Read1 Name, ResultDir [File], Static Report [Link], Demux Report [Link], Denoising stats [Link], Feature Table [Link], Rep Seqs Report [Link], Taxonomy Barplot [Link], Taxonomy List [Link], Shannon Diversity [Link], Jaccard Diversity [Link], Bray Curtis Diversity [Link], Jaccard Emperor Plot [Link], Bray Curtis Emperor Plot [Link], Alpha rarefaction [Link], Differential abundace [Link]
Samsa2App Metatranscriptomics pipeline with Samsa2. Name, Read1 Name, annotationFileRefSeq [File], annotationORGFileRefSeq [File], annotationFUNCFileRefSeq [File]
VirDetectApp Virome analysis for the diagnostic use in veterinary virology
Name, Read1, Species Name, Species, virBuild, hostBuild, paired, Read Count, OutDir [File], OutReport [Link]
CountSpacerApp QC Tool for sgRNA libraries. Name, Read1 Name, Report [File], Html [Link], Count [File], Species, Read Count
MageckCountApp Name, Read1 Name, Count [File], Log [File], Read Count, libName, Species
NestLinkApp NestLink - an R data package to guide through Engineered Peptide Barcodes for In-Depth Analyzes of Binding Protein Ensembles - https://bioconductor.org/packages/release/data/experiment/html/NestLink.html Name, Read1, FlashLog Name, NestLink Result [File]
MACS2App Capturing the influence of genome complexity to evaluate the significance of enriched ChIP regions
Name, BAM, BAI, refBuild Name, Species, refBuild, refFeatureFile, paired, CalledPeaks [File], BED [File], PeakSequences [File], BigWigFile [File], BAM [File], BAI [File]
ConvERDSApp This converts the result DataSet of EAGLERCApp to an input DataSet of DNAHaplotypeCallerGVCFApp. Name, Species, dummy Name, BAM, refBuild, Species, Dummy [File]
EAGLERCApp EAGLE: Explicit Alternative Genome Likelihood Evaluator
Name, BAM1, BAM2, refBuild1, refBuild2, Species Name, Parent1RefBAM [File], Parent1AltBAM [File], Parent1UnkBAM [File], Parent1MulBAM [File], refBuild1, Parent2RefBAM [File], Parent2AltBAM [File], Parent2UnkBAM [File], Parent2MulBAM [File], stdout log [File], errout log [File], refBuild2, Species, dummy [File]
MergeDataSetApp Merging two DataSets

Name, BAM, refBuild, Species Name, BAM1, BAM2, refBuild1, refBuild2, Species, dummy [File]
FlashApp Fast Length Adjustment of SHort reads Name, Read1, Read2 Name, Read1 [File], Log [File], Species, Read Count
MergeRunDataApp Merging fastq files from two illumina runs by name

Name, Species, Read1 Name, Result [File]
EnaApp Download public data from ENA
Name, projectID Name, projectID, ENA Result [File]
BuscoApp BUSCO: from QC to gene prediction and phylogenomics Name, Draft Name, BuscoPlot [Link], BuscoOut [File], Species
CountQCApp Quality control after counting reads
Name, Count, Species, refBuild, featureLevel, refFeatureFile Name, Species, refBuild, Static Report [Link], Live Report [Link], Report [File]
CrisprScreenQCApp Screens of CRISPR samples for contaminations Name, Read1 Name, Report [File], Html [Link]
DnaBamStatsApp Runs the following tools that check alignment statistics for the DNA applications
Name, BAM Name, Samstat Result [File], Qualimap Result [File], Picard Result [File], Samstat Report [Link], Qualimap Report [Link], Picard Report [Link]
DnaQCApp Quality control after the alignment of RNAseq reads
Name, BAM, BAI, refBuild, Species Name, Report [File], Html [Link], Species, refBuild, refFeatureFile
ExceRptReportApp QC report of exceRpt outputs. Name, excerpt, Species, refBuild Name, Species, refBuild, Static Report [Link], Report [File]
ExploreMageckCountsApp Quality control after counting sgRNAs with Mageck
Name, Count, Species Name, Species, libName, Static Report [Link], Live Report [Link], Report [File]
FastqScreen10xApp Screen files for contaminations or ribosomal RNA content
Name, RawDataDir, Read Count Name, Report [File], Html [Link]
FastqScreenApp Screen files for contaminations or ribosomal RNA content
Name, Read1, Read Count Name, Report [File], Html [Link]
Fastqc10xApp A quality control tool for NGS reads
Name, RawDataDir, Read Count Name, Report [File], Html [Link], MultiQC [Link]
FastqcApp A quality control tool for NGS reads
Name, Read1 Name, MultiQC Report [Link], MultiQC [File], FastQC [File]
GceApp GCE(genomic charactor estimator): a bayes model based method to estimate the genome size, genomic repeat content and the heterozygsis rate of the sequencing sample
Name, Read1 Name, GCE Result [File], Report [Link]
MageckTestApp Run test module in the tool Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK) Name, Count Name, Report [File], Report [Link]
NanoPlotApp NanoPlot: Plotting tool for long read sequencing data and alignments
Name, Read1 Name, NanoPlot Result [File], Report [Link]
PreqcApp Preqc - Illumina read pre-assembly quality control and data exploration module within sga Name, Read1, Read2 Name, PreqcReport [Link], PreqcReport [File], PreqcOut [File]
QuastApp QUAST (Quality Assessment Tool for Genome Assemblies) Name, Draft Name, QuastReport [Link], QuastOut [File], Species
RnaBamStatsApp Quality control after the alignment of RNAseq reads
Name, BAM, BAI, refBuild, Species Name, Report [File], Html [Link], Species, refBuild, refFeatureFile
RnaBiasApp Name, Read1, Species Name, Report [File], Report [Link]
BDRhapsodySAApp This wrapper runs a CWL workflow for the analysis of BD Single-Cell Multiomics. Name, Read1, Read2, Species Name, Species, refBuild, refFeatureFile, featureLevel, transcriptTypes, SCDataOrigin, ResultDir [File], Report [Link], CountMatrix [Link], UnfilteredCountMatrix [Link], Read Count
CellBenderApp CellBender is a software package for eliminating technical artifacts from high-throughput single-cell RNA sequencing (scRNA-seq) data. ToolLink: https://github.com/broadinstitute/CellBender
Name, Species, refBuild, refFeatureFile, CountMatrix Name, Species, refBuild, refFeatureFile, Static Report [Link], ResultDir [File], CountMatrix [Link], UnfilteredCountMatrix [Link]
CellRangerARCApp This wrapper runs cellranger-arc count in Single-library analysis mode. Name, RNADataDir, ATACDataDir, Species Name, Species, refBuild, refFeatureFile, featureLevel, ResultDir [File], Report [Link], CountMatrix [Link], UnfilteredCountMatrix [Link], Read Count
CellRangerATACApp This wrapper runs cellranger atac count in Single-library analysis mode. Name, RawDataDir, Species Name, Species, refBuild, refFeatureFile, featureLevel, ResultDir [File], Report [Link], CountMatrix [Link]
CellRangerAggrApp This wrapper runs cellranger aggr in multi-library analysis mode. Name, CountMatrix Name, Species, refBuild, refFeatureFile, featureLevel, transcriptTypes, ResultDir [File], Report [Link], CountMatrix [Link]
CellRangerApp This wrapper runs cellranger count in Single-library analysis mode. Name, RawDataDir, Species Name, Species, refBuild, refFeatureFile, featureLevel, transcriptTypes, SCDataOrigin, ResultDir [File], Report [Link], CountMatrix [Link], UnfilteredCountMatrix [Link], Read Count
CellRangerMultiApp This wrapper runs cellranger multi in Single-library analysis mode.

Name, RawDataDir, Species Name, Species, refBuild, refFeatureFile, featureLevel, transcriptTypes, SCDataOrigin, ResultDir [File,Link], Read Count
MergeSCDataSetsApp Merging more than two DataSets generaged from same library for CellRangerApp

Name, Species, RawDataDir Name, Species
ONTwfScApp A research pipeline designed to identify the cell barcode and UMI sequences present in nanopore sequencing reads generated from single-cell gene expression libraries
Name, Read1 Name, OutDir [File], OutReport [Link]
SCFeatBarcodingApp Single cell report
Name, Species, refBuild, CountMatrix, ResultDir Name, Species, refBuild, refFeatureFile, Static Report [Link], Report [File]
SCTrajectoryInferenceApp Trajectory inference analysis for single cell data
Name, Static Report Name, Report [File], Static Report [Link]
STARsoloApp This wrapper runs STARsolo in Single-library analysis mode. Note that it only runs on Single Cell GEX 10X libraries. Name, RawDataDir, Species Name, Species, refBuild, refFeatureFile, featureLevel, soloFeatures, transcriptTypes, ResultDir [File], CountMatrix [Link], UnfilteredCountMatrix [Link]
ScSeuratApp Single cell report
Name, Species, refBuild, CountMatrix, ResultDir, Condition Name, Species, refBuild, refFeatureFile, Static Report [Link], SC Cluster Report [File], SC Seurat
ScSeuratCombineApp The report of merged single cell samples/plates
Name, Species, refBuild, refFeatureFile, Static Report Name, Species, Static Report [Link], Report [File]
ScSeuratCombinedLabelClusters The report of merged single cell samples/plates
Name, Species, Static Report, Report Name, Species, Static Report [Link], Report [File]
ScSeuratCompareApp Empirical analysis of digital gene expression data in R
Name, Report Name, Static Report [Link], Report [File]
ScSeuratFilterClustersApp Single cell report
Name, Species, refBuild, SC Seurat Name, Species, refBuild, refFeatureFile, Static Report [Link], SC Cluster Report [File], SC Seurat
ScSeuratLabelClustersApp Single cell report
Name, Species, refBuild, SC Seurat Name, Species, refBuild, refFeatureFile, Static Report [Link], SC Cluster Report [File], SC Seurat
VelocytoApp This wrapper runs velocyto in Single-library analysis mode. Name, ResultDir Name, LOOM [File], Species, refBuild, refFeatureFile, featureLevel, Read Count
SpaceRangerAggrApp This wrapper runs spaceranger aggr in multi-library analysis mode. Name, CountMatrix Name, Species, refBuild, refFeatureFile, featureLevel, transcriptTypes, ResultDir [File], Report [Link], CountMatrix [Link]
SpaceRangerApp This wrapper runs space ranger count in Single-library analysis mode. Name, RawDataDir, Species, Slide, Area Name, Species, refBuild, refFeatureFile, featureLevel, transcriptTypes, ResultDir [File], Report [Link], CountMatrix [Link], Read Count, Count [Link]
SpatialSeuratApp Single cell report
Name, Species, refBuild, CountMatrix, ResultDir Name, Species, refBuild, refFeatureFile, Static Report [Link], Report [File]
SpatialSeuratSlidesApp Combine multiple slides from Visium/plates
Name, Species, refBuild, refFeatureFile, Static Report Name, Species, Static Report [Link], Report [File]
WordCountApp test applicaiton Supercalifragilisticexpialidocious!! Name, Read1 Name, Stats [File], Options
GATKJointGenotypingByGenomicsDBApp Genotyping by GenomicsDBImport,GenotypeGVCFs, and hard-filtering by VariantFiltration Name, GVCF, GVCFINDEX, Species, refBuild, Dummy Name, Raw VCF [File], Filtered VCF [File], Species, refBuild
GATKv4DNAHaplotypeCallerGVCFApp Haplotype calling for DNA-seq with > version 4.0 in GVCF mode
Name, BAM, refBuild, Dummy Name, GVCF [File], GVCFINDEX [File], Species, refBuild, Dummy [File]
GATKv4FilteringSNPsByReferenceVCFApp filtering out SNPs by the VCF coming from reference accession
Name, Raw VCF, Filtered VCF, Species, refBuild Name, Filtered VCF [File], Species, refBuild, Script [File], Script log [File]
GATKv4GVCF2FilteredVCFApp genotype,merge and annotate gvcf-Files
Name, GVCF, GVCFINDEX, Species, refBuild, Dummy Name, Raw VCF [File], Filtered VCF [File], Species, refBuild
GATKv4JointGenoTypesApp genotype,merge and annotate gvcf-Files
Name, GVCF, GVCFINDEX, Species, refBuild, Dummy Name, Raw VCF [File], Filtered VCF [File], Species, refBuild
GatkDnaHaplotyperApp Haplotype calling for DNA-seq
Name, BAM, BAI, refBuild Name, GVCF [File], GVCFINDEX [File], Species, targetFile, refBuild
GatkJoinGenoTypesApp genotype,merge and annotate gvcf-Files
Name, GVCF, GVCFINDEX, Species, refBuild Name, Report [File], Species, refBuild
GatkRnaHaplotyperApp Haplotype calling for RNA-seq
Name, BAM, BAI, refBuild Name, GVCF [File], GVCFINDEX [File], Species, refBuild
MpileupApp Variant analysis with samtools/bcftools.
Name, BAM, BAI, refBuild, Species Name, VCF [File], TBI [File], Report [File], Html [Link], Species, refBuild
PbsvApp pbsv - PacBio structural variant (SV) calling and analysis tools
Name, BAM, BAI, refBuild Name, refBuild, OutDir [File], OutReport [Link]